heparinase

Heparinase I (heparin lyase I) cleaves heparin and to a lesser extent heparan sulfate (ratio approx. 3:1).
Usage
Neutralization of heparin in blood and plasma samples before analysis (as an additive to or contained in collection tubes).
As a component of in vitro diagnostic tests for clotting evaluations and platelet function tests for removal of heparin interference.
Preparation of low molecular weight heparins from unfractionated heparin.
Research reagent (glycobiology, preparation of oligosaccharide libraries, preparation of dissaccharides from heparin).
General Information
Synonyms：Heparinase; heparin lyase I; heparin eliminase

Gene Source: Native Flavobacterium heparinum

EC Number: 4.2.2.7

CAS Number: 9025-39-2

MDL Number: MFCD00131317

Catalyzed Reaction: Heparinase I cleaves selectively, via an elimination mechanism, highly sulfated polysaccharide chains containing 1-4 linkages between hexosamines and O-sulfated iduronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme also cleaves the antithrombin III binding pentasaccharide domain in the heparin molecule.

Substrate Specificity: Heparin; heparan sulfate (specific activity with heparin is approx. 3 times higher than with heparan sulfate).

Properties
Molecular weight: 42.8 kDa

Isoelectric point: 9.3 – 9.5

pH optimum for activity: 7.4

pH range for activity: 4 – 9

Optimal temperature range: 20 ℃ – 37 ℃

Purity: ≥ 98 % by reversed phase HPLC analysis

Protein impurities: < 0.1%

Specific Activity: > 400 IU/mg (max. 417 IU/mg)

Concentration: 10 IU/mL

Unit Definition: One international unit (IU) of heparinase I is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from porcine mucosal heparin per minute at 35 ℃ and pH 7.0.

Stability: Expiration of heparinase I is 12 months from manufacturing date, frozen at -20 ℃ in PBS.

Applications
Heparinase I is useful in many applications (usually with heparinase II and heparinase III), for example:

For the neutralization of heparin in blood and plasma samples before analysis.
For similar in vitro neutralization of low molecular weight heparins.
As integral part of in vitro diagnostic tests for the neutralization of heparin (blood clotting tests, platelet tests).
In blood collection tubes for the neutralization of heparin.
For the preparation of low molecular weight heparins from unfractionated heparin.
As a research reagent (glycosaminoglycan degradation)
For the preparation of disaccharides of heparin and the preparation of oligosaccharide libraries.