β-agonist ELISA kit

β-agonists ELISA Test Kit
Catalog No. LSY-10036
1. Principle
The GreenSpring®β-agonists test kit is based on the competitive enzyme immunoassay for the detection of β-agonists in the urine, pork and other samples. The coupling antigen is pre-coated on the micro-well stripes. The β-agonists in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-β-agonists antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the β-agonists in the sample. This value is compared to the standard curve and the β-agonists residues is subsequently obtained.

2. Technical specifications
Sensitivity: 0.2 ppb
Detection limit:
Urine 0.2 ppb
Tissue (Method one) 0.8 ppb
Tissue (Method two) 0.2 ppb
Feed 2 ppb
Recovery rate:
Urine, serum 95%±10%
Tissue 80%±10%
Feed 80%±15%
Cross-reaction rate:
Clenbuterol 100%
Salbutamol 120%
Bromchlorbuterol 130%
Brombuterol 90%
Mabuterol 70%
Clenpenterol 65%
Mapenterol 55%
Tebutalin 42%
Carbuterol 35%
Clenproperol 15%
Cimaterol 12%
Dobutamine <3%
Ritodrine <1%
Ractopamine <0.5%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.2ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb
3） Enzyme conjugate (12 mL) red cap
4） Antibody working solution (10 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap