Tetracyclines(TCs) ELISA Test Kit

Tetracyclines(TCs) ELISA Test Kit
Catalog No. LSY-10006
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Tetracyclines in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tetracyclines in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tetracyclines antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tetracyclines in it. This value is compared to the standard curve and the Tetracyclines concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.05 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue (chicken):
Tetracyclines 0.4ppb
Minocycline 0.4ppb
RoliTetracyclines 0.4ppb
Aureomycin 0.4ppb
Demethylchlor Tetracyclines 1.2ppb
Terramycin 0.8ppb
Doxycycline 1ppb
Pork, Honey
Tetracyclines 0.5ppb
Minocycline 0.5ppb
RoliTetracyclines 0.5ppb
Aureomycin 0.5ppb
Demethylchlor Tetracyclines 1.5ppb
Terramycin 1ppb
Doxycycline 1.2ppb
Cross-reaction rate:
Tetracyclines 100%
Minocycline 125%
RoliTetracyclines 110%
Aureomycin 100%
Demethylchlor Tetracyclines 35%
Terramycin 58%
Doxycycline 45%
Recovery rate:
Chicken, Pork, honey 85±20%
3. Components
1 Micro-well strips 12 strips with 8 removable wells each
2 Standard Concentrate (81ppb) 1ml black cap
3 High-concentration standard (100ppb) 1ml black cap
4 Enzyme conjugate 7ml white cap
5 Antibody working solution 7ml white cap
6 Substrate A 7ml white cap
7 Substrate B 7ml black cap
8 Stop solution 7ml yellow cap
9 20× concentrated washing buffer 15ml white cap
10 10× sample extract 50ml * 2 transparent cap
11 Standard redissolving solution 15ml *2 blue cap