Swine Foot And Mouth Disease IgG Antibody ELISA Test kit

Swine Foot and Mouth Disease IgG Antibody ELISA Test kit
Catalog No. LSY-30002
1. Introduction
The GreenSpring™ FMD-Ab ELISA is developed to detect the O type antibodies of Porcine Foot and Mouth Disease in porcine serum samples and assess the immunity conditions against FMD in the pig farms.

2. Principle
The kit is based on an indirect enzymatic immunoassay.The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific peroxidase conjugate(IgG-HRP). After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer(630 nm).

3. The kit components
1 FMD coated microplate 2 pieces (96 wells)
2 FMD Negative control serum (green lid) 1 tube (1.5 mL)
3 FMD Positive control serum (red lid) 1 tube (1.5 mL)
4 FMD Enzyme conjugate (yellow lid) 1 bottle (22 mL)
5 20×concentrated washing buffer (white lid) 1 bottle (50 mL)
6 Substrate A (purple lid)/B (yellow lid) (12 mL/each)
7 Stop solution (blue lid) 1 bottle (12 mL)
8 Sample diluent (transparent lid) 1 bottle (50 mL)
9 Adhesive Foil 6 pieces
10 Instruction 1 piece
(Note: Never mix up reagents from test kits in different batch number)