Sulfonamides (SAs) ELISA Kit

Sulfonamides (SAs) ELISA Kit
Catalog No. LSY-10009
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Sulfonamides residue. The coupling antigens are pre-coated on the micro-well stripes. The Sulfonamides in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Sulfonamides antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfonamides in the sample. This value is compared to the standard curve and the Sulfonamides concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Tissue (high-detection-limit method) 0.4 ppb
Tissue (lower-detection-limit method)……………………………………...5 ppb
Honey 1 ppb
Serum, urine 4 ppb
Milk 20 ppb
Cross-reaction rate
Sulfamerazine (SM1) 100%
Sulfadiazine (SD or SDZ) 130.2%
Sulfamonomethoxine (SMM) ……………………………………………..181.8%
Sulfamethoxydiazine(SMD) 194.8%
Sulfanethazine(SM2)………………………………………………………. 89.3%
Sulfisomidine (SM2′) …………………………………………………..… 70.6%
Sulfadimethoxine(SDM)…………………………………………………… 140.8%
Sulfadoxine (SDM ′) …………………………………………………….. 132.1%
Sulfamethoxazole (SMZ) ….. 86.6%
Sulfadimoxine (SDM2) ……………………………………………………. 144.7%
Phthalylsulfathiazole (PST) 73.9%
Sulfamethizole (SMT) ……………………………………………………… 69.4%
Sulfafurazole(SIZ) 76.5%
Sulfathiazole (ST) 103.3%
Sulfaquinoxaline(SQX)………………………………………………………59.4%
Sulfamethoxypyridazine (SMP)……………………………………………108.6%
Sulfaclozine (Esb3) ………………………………………………………… 72.1%
Sulfachloropyridazine (SCPA)……………………………………………… 59.6%
Sulfabenzoyl (SML)…………………………………………………………..104%
Conclusion drawn from the cross-reaction rate: This kit can be used for the test of Sulfonamides, totally meet the national testing demand of Sulfonamides.
Recovery rate
Tissue, urine, milk 85±25%
Honey, serum 80±23%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 1ppb, 3ppb, 9ppb, 27ppb and 81ppb
3） Enzyme conjugate (7 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 20× concentrated redissolving solution (50 mL) transparent cap