Streptomycin ELISA Test Kit

Streptomycin ELISA Test Kit
Catalog No. LSY-10024-2
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Streptomycin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Streptomycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti- Streptomycin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Streptomycin concentration in the sample. This value is compared to the standard curve and the Streptomycin concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.1 ppb
Incubation Temperature: 37℃
Incubation Time: 30min—30min—15min
Detection limit:
Chicken 1 ppb
Chicken liver, milk 4 ppb
Honey, Royal jelly 2 ppb
Recovery rate:
Milk 85±22%
Chicken 80±17%
Honey, Royal jelly 75±19%
Cross-reaction rate:
Streptomycin 100%
Dihydrostreptomycin 108%
Kalamycin <0.1%
Gentamycin <0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.4 ppb, 1.6 ppb, 6.4 ppb, 25.6 ppb
3） Enzyme conjugate (12 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 10× concentrated redissolving solution (50 mL) transparent cap