Salbutamol ELISA Test Kit

Salbutamol ELISA Test Kit
Catalog No. LSY-10014
1. Principle
The test kit is based on the competitive enzyme immunoassay for the detection of Salbutamol in urine, tissue etc. The coupling antigen is pre-coated on the micro-well stripes. The Salbutamol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Salbutamol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Salbutamol in it. This value is compared to the standard curve and the Salbutamol residues is subsequently obtained.

2. Technical specifications
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Urine……………………………………………………………..…..…..….0.1 ppb
Tissue (method one) 0.4 ppb
Tissue (method two) 0.1 ppb
Feed 1 ppb
Recovery rate
Urine ………………………………………………………………………95%±10%
Tissue 80±15%
Feed 85±15%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.1ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb,8.1 ppb
3） Enzyme conjugate (7 mL) red cap
4） Antibody working solution (10 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 2× concentrated redissolving solution (50 mL) ……………………..white cap
9） 20x concentrated washing buffer (40 mL) transparent cap
10）20x Sample extraction solution (50 mL) ……………….………..yellow cap