Ractopamine ELISA Test Kit

Ractopamine ELISA Test Kit
Catalog No. LSY-10013
1. Principle
The test kit is based on the competitive enzyme immunoassay for the detection of Ractopamine in the sample. The conjugated antigens is pre-coated on the micro-well stripes. The Ractopamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Ractopamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Ractopamine concentration in the sample. The value is compared to the standard curve and the Ractopamine concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.05 ppb
Detection limit
Urine 0.1 ppb
Tissue……………………………………………………………………… 0.2 ppb
Meat 0.1 ppb
Liver 0.1 ppb
Feed 0.2 ppb
Recovery rate
Urine 95±10%
Tissue 90±10%
Feed 90±10%
Cross-reactions
Ractopamine 100%
Dobutamine 1%
Clenbuterol <0.1%
Salbutamol <0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15ppb, 0.45 ppb, 1.35 ppb, 4.05 ppb
3） Enzyme conjugate (7 mL) red cap
4） Antibody working solution (10 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap