Quinolones(QNS) ELISA Test Kit

Quinolones(QNS) ELISA Kit
Catalog No. LSY-10016
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Quinolones in the chicken, pork, milk, honey, egg, etc. The coupling antigens are pre-coated on the micro-well stripes. The Quinolones in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Quinolones antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Quinolones in the sample. This value is compared to the standard curve and the Quinolones concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 1 ppb
Cross-reaction rate:
Enrofloxacin (ENR) 100%
Ciprofloxacin (CIF) 92.88%
Ofloxacin (OFL) 98.86%
Aosuo Li acid (OXO) 116.86%
Danofloxacin (DAN) 102.63%
Norfloxacin (NOR) 148.65%
Lomefloxacin (LOM) 86.24%
Pefloxacin (PEF) 156.60%
Enoxacin (ENO) 98.97%
Fluoroquinolone acid (FLU) 96.73%
Ma Paul Sand Star (MAR) 110.63%
Ammonia difloxacin (AMI) 98.86%
That difloxacin (NAD) 56.81%
Recovery rate
Tissue 80±15%
Serum 80±15%
Honey 75±15%
Detection limit
Enrofloxacin (ENR)………………………………………………………….1 ppb
Ciprofloxacin (CIF) …………………………………………………..about 1 ppb
Ofloxacin (OFL) …………………………………………………… .about 1 ppb
Danofloxacin (DAN) …………………………………………………about 1 ppb
Norfloxacin (NOR) …………………………………………………about 0.5 ppb
Lomefloxacin (LOM) …………………………………………………about 1 ppb
Pefloxacin (PEF) ……………………………………………………about 0.5 ppb
Enoxacin (ENO) ………………………………………………………about 1 ppb
Aosuo Li acid (OXO) …………………………………………………about 1 ppb
Fluoroquinolone acid (FLU) …………………………………………about 1 ppb
Ma Paul Sand Star (MAR) ………………………………………..…about 1 ppb
Ammonia difloxacin (AMI) ……………………………………………about 1 ppb
That difloxacin (NAD) …………………………………………………about 2 ppb

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 1 ppb, 3 ppb, 9 ppb, 27 ppb and 81 ppb
3） Enzyme conjugate (7 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 2× concentrated redissolving solution (50 mL) transparent cap