Porcine Streptococcus suis (S.suis) Antibody ELISA Test Kit

Porcine Streptococcus suis (S.suis)
Antibody ELISA Test Kit
Catalog No. LSY-30019
1. Brief
The GreenSpring® Porcine Streptococcus suis antibody test kit is developed to detect all subtype antibodies of Porcine Streptococcus suis Disease in porcine serum samples and assess the immunity conditions against S.suis in the pig farms.
2. Principle
The GreenSpring® Porcine S.suis antibody ELISA test kit is made from the antigen coated microtiter plate(coated with GDH antigen) and other reagents. It applies the indirect ELISA principle to test the antibodies against S.suis in porcine serum. In the test, the coated antigen combine with GDH-IgG in serum, then add IgG-GDH to specifically bind with complex of antibody-antigens on the microplate. With the TMB substrate, it will generate an amount of color. The depth of color is relative with the content of the GDH-IgG, when the value of color is greater than the cut-off value, the pigs are vaccinated well.
3. Components
1 GDH coated microplate 2 pieces (96 wells)
2 Negative control serum (green lid) 1 tube (1.5 mL)
3 Positive control serum (red lid) 1 tube (1.5 mL)
4 Enzyme conjugate (yellow lid) 1 bottle (22 mL)
5 20×Wash Concentrate (white lid) 1 bottle (50 mL)
6 Substrate A (purple lid)/B (yellow lid) 1 bottle each (12 mL/bottle)
7 Stop solution(yellow lid) 1 bottle (12 mL)
8 Sample diluent solution(transparent lid) 1 bottle (25 mL)
9 Adhesive Foil 6 pieces
10 Instruction 1 piece
4. Material Required But not Provided
1Microplate Reader (single wave length: 450 nm or double wave length 450/630 nm).
2Microplate Washer.
3Micropipettes, adjustable.
4Constant temperature box or incubator.
5Oscillator.
5. Sample requirement
1The samples are porcine serum, which should be collected with no bacteria. The storage time should be less than 1 week at 2-8 ℃, if for long term, it should be kept at -20℃.
2Avoid to use the samples with severe hemolysis, precipitate, contaminated by bacteria or protein suspension.
3The EDTA, heparin sodiun and other anticoagulants will not affect the results.