Peste des petits ruminants virus (PPRV) antibody ELISA test kit for ovine

Peste des petits ruminants virus (PPRV) antibody ELISA test kit for ovine
Catalog No. LSY-30035
1.Principle
This kit is used to detect specific antibody against Peste des petits ruminants virus (PPRV) Antibody in serum qualitatively, for monitoring antibody after PPRV immune and serological diagnosis of infection in sheep.
This kit use indirect ELISA method, pured Ovine PPRV antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is PPR virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-PPR virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.
2. Reagents and contents
Code Item Spec. Code Item Spec.
1 Antigen Coated plates
96 wells 1/2 plate 7 Stop solution 6/11 ml
2 Enzyme Conjugate 6/11 ml 8 Positive control 0.8/1.6 ml
3 10X Washing buffer 50 ml 9 Negative control 0.8/1.6 ml
4 Substrate A 6/11 ml 10 Serum dilute plate 1/2 plate
5 Substrate B 6/11 ml 11 Adhesive Foil 2/4 pieces
6 Sample diluent 50 ml 12 Instruction 1 piece
3. Material required not provided
1) Micropipette: 0.5-10ul, 10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells with 450/630nm wavelenth.
5) Distilled water or deionized water.
6) Microplate Washer
4. Sample preparation
Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis