Oxytetracyline ELISA Test Kit

Oxytetracyline ELISA Test Kit
Catalog No. LSY-10044
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Oxytetracyline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Oxytetracyline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Oxytetracyline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Oxytetracyline in it. This value is compared to the standard curve and the Oxytetracyline concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.4 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue about 6ppb
Cross-reaction rate:
Oxytetracyline 100%
Tetracyclines 200%
Chlortetracycline 200%
Doxycycline 20%
Recovery rate:
Tissue 90±15%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 10× concentrated standard solution (0.5ml/bottle): 0 ppb, 4ppb, 12ppb, 36ppb, 108ppb
3） Enzyme conjugate (6 mL)
4） Antibody working solution (6 mL)
5） Substrate A (6 mL)
6） Substrate B (6 mL)
7） Stop solution (7 mL)
8） 20× concentrated washing buffer (30 mL)
9） 20× concentrated redissolving solution (10 mL)