Ochratoxin A（OTA）ELISA Test Kit

Ochratoxin A（OTA）ELISA Test Kit
Catalog No. LSY-10034
1. General
Ochratoxin A are secondary metabolites of Aspergillus and Penicillium strains, exist widely in grain and grain products, spices and coffee beans and other products. Ochratoxin A has a strong liver and kidney toxicity, and has teratogenic, mutagenic and carcinogenic effects, which is serious harmful to human health.
Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) has been a common method of Ochratoxin A detection, but sample preparation and samples analysis of this method is time-consuming and exhausting. The use of Ochratoxin A ELISA kit to analyze Ochratoxin A residue of samples is fast and accurate.
This kit is a new generation of fungal toxins detection products in application of ELISA technology, to minimize operating errors and work intensity.

2. Principle
The test is based on a direct competitive enzyme immunoassay method. The microtiter wells are coated with Ochratoxin antigen. Free Ochratoxin in sample solutions and the immobilized antigen compete for Ochratoxin enzyme conjugate.
While Ochratoxin antibody and HRP secondary antibody (HRP material) combined, via the TMB substrate show color, sample absorbance values of Ochratoxin has a negative correlation with its content, compared with the standard curve and then multiplied by the corresponding the dilution factor, can draw Ochratoxin content in samples.

3. Application
This test kit can be used to detect Ochratoxin in Corn, wheat and other grain samples qualitatively and quantitatively.

4. Technical specifications
Sensitivity: 0.1 ppb
Detection limit:
Corn, whole wheat …………………………………………………1ppb
Recovery rate:
Corn ……………………………………………………………….80±10%
Whole wheat………………………………………………….…..90±10%
Cross-reaction rate
Ochratoxin A …………………………………………………………100%
Ochratoxin B …………………………………………………………9.3%
Ochratoxin C ………………………………………………………….46%
Coefficient of variation: <10%

5. Components
1) OTA Micro-well strips: 12 strips with 8 removable wells each
2) 5 x Concentrated standard (3x) …………………………….1 ml each
(Remark: Concentrated standard (3x) is 0 ppb (zero standard), 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb; Working
Standard is 0ppb, 0.1ppb, 0.3ppb, 0.9ppb, 2.7ppb)
3) OTA Enzyme-labled conjugate .............................................. 12 ml
4) OTA Antibody ........................................................................... 6ml
5) Substrate A .............................................................................. 6ml
6) Substrate B................................................................................ 6ml
7) Stop solution............................................................................... 6ml
8) Concentrated wash buffer（10×）...........................................40ml