Nitrofuran (AOZ) ELISA Test Kit

1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of AOZ in the samples. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with AOZ concentration in the sample. This value is compared to the standard curve and the content of the corresponding AOZ is subsequently obtained.

2. Technical specifications
Sensitivity: 0.02 ppb
Detection limit:
Tissue (Chicken/duck/fish/shrimp/pork/beef/mutton) 0.04 ppb
Liver (Chicken/duck/pork/beef/mutton) 0.04 ppb
Honey, milk, intestine, urine 0.04 ppb
Shrimp, fish (some interference) 0.2 ppb
Recovery rate:
Tissue, liver 90±10%
Honey, milk, intestine, urine 75±15%
Cross-reaction rate:
AOZ 100%
AHD <0.1%
AMOZ <0.1%
SEM <0.1%

3. Components
1. Micro-well strips: 12 strips with 8 removable wells each
2. 6× standard solution (1 mL each): 0 ppb, 0.02 ppb, 0.06 ppb, 0.18 ppb, 0.54 ppb and 1.62 ppb
3. Enzyme conjugate (12 mL) red cap
4. Antibody working solution (7 mL) blue cap
5. Substrate A solution (7 mL) white cap
6. Substrate B solution (7 mL) black cap
7. Stop solution (7 mL) yellow cap
8. 20× concentrated washing buffer (40 mL) white cap
9. 2× concentrated redissolving solution (50 mL) transparent cap
10. 2-Nitrobenzaldehyde(C7H5NO3)( 10 mL) white cap