Neomycin ELISA test kit

Neomycin ELISA test Kit
Catalog No. LSY-10005
1. Principle
This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Neomycin in it. This value is compared to the standard curve and the Neomycin concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.5 ppb
Detection limit
Tissue 10 ppb
Honey 10 ppb
Milk, milk powder 10 ppb
Recovery rate
Chicken, pork, honey, fish, shrimp 85±15%
Liver (chicken or porcine) 70±10%
Milk, milk powder ………………………………………………………….. 85±10%
Cross-reaction rate
Neomycin 100%
Streptomycin < 0.1%
Gentamicin < 0.1%
Dihydrostreptomycin < 0.1%
Kanamycin < 0.1%
Tobramycin < 0.1%
Sisomicin < 0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb
3） Enzyme conjugate (7 mL) red cap
4） Antibody concentrated solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 2× concentrated redissolving solution (50 mL) transparent cap