Metronidazole ELISA Test Kit

Metronidazole ELISA Test Kit
Catalog No. LSY-10048
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Metronidazole in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Metronidazole in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Metronidazole antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Metronidazole in it. This value is compared to the standard curve and the Metronidazole concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1ppb
Detection limit
Pork, Chicken about 0.5ppb
Honey about 0.1ppb
Raw milk about 1ppb
Raw egg (method 1) about 0.5ppb
Raw egg (method 2) about 1.5ppb
Cooked egg about 1ppb
Note: ppb= ng/ml or ng/g
Cross-reaction rate
Metronidazole 100%
Hydroxyl metronidazole 47%
Hydroxymethyl dimetridazole 53%
Tinidazole 2%
Dimetridazole 800%
Ornidazole 5%
Recovery rate
90±30%
3. Components
1）Micro-well strips: 12 strips with 8 removable wells each
2）6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb
3）Enzyme conjugate (12 mL) 1 bottle
4）Antibody working solution (6mL) 1 bottle
5）Substrate A solution (6 mL) 1 bottle
6）Substrate B solution (6 mL) 1 bottle
7）Stop solution (6 mL) 1 bottle
8）20× concentrated washing buffer (30 mL) 1 bottle
9）Redissolving solution (50 mL) 1 bottle
10）Tissue sample treatment solutionⅠ(dry powder, optional) 1 bottle