Melamine ELISA test kit

Melamine ELISA Test Kit
Catalog No. LSY-10026
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 1 ppb
Detection limit:
Milk powder 20 ppb
Milk 16 ppb
Milk/Milk powder (Method two) 1 ppb
Tissue (chicken, pork, duck, fish, shrimp and liver) 2 ppb
Feed 100 ppb
Egg 20 ppb
Serum 4 ppb
Recovery rate:
Milk/Milk powder 90±15%
Tissue 85±10%
Feed 85±10%
Egg 80±10%
Cross-reaction rate:
Melamine 100%
Cyanuric acid 60%
Triazine <1%
Diamino atrazine <1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb,1 ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb
3） Enzyme conjugate (7 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) white cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 2× concentrated redissolving solution (50 mL) transparent cap