Malachite green ELISA kit

Malachite green ELISA kit
Catalog No. LSY-10027
1. Principle
This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. This value is compared to the standard curve and the content of Malachite green residues is subsequently obtained.

2. Technical specifications
Sensitivity: 0.025 ppb
Incubator temperature: 25℃
Incubator time: 30min～30min～15min
Detection limit
Aquatic products e.g. Shrimp, Fish 0.05 ppb
Water 0.05 ppb
Recovery rate
Shrimp, Fish, water 75±18%
Cross-reaction rate
Malachite green 100%
Leucomalachite green 0.1%
Gentian violet 95%
Leucocrystal violet 0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） Standard concentrated solution (1 mL) 100 ppb
3） Enzyme conjugate (12 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL)……………………………………………. black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） Reagent A (50 mL) transparent cap
10) Reagent B (50 mL) blue cap