Human Hepcidin(Hepc) ELISA Kit

Intended Use:
This kit is used to assay the Hepcidin(Hepc) in the sample of human’s serum, blood plasma, and other related tissue Liquid.
Test principle
The kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Hepcidin(Hepc) in samples. Add Hepcidin(Hepc) to monoclonal antibody Enzyme well which is pre-coated with Human Hepcidin(Hepc)monoclonal antibody, incubation; then, add Hepcidin(Hepc)antibodies labeled with biotin, and combined with Streptavidin-HRP to form immune complex; then carry out incubation and washing again to remove the uncombined enzyme. Then add Chromogen Solution A, B, the color of the liquid changes into the blue, And at the effect of acid, the color finally becomes yellow. The chroma of color and the concenthumanion of the Human Substance Hepcidin(Hepc) of sample were positively correlated.