Foot and mouth disease virus (FMDV) Type A antibody ELISA test kit for bovine and ovine

Foot-and-mouth disease virus (FMDV) Type A Antibody ELISA Test Kit
For Bovine
Catalog No. LSY-30034-1
1.Principle
This kit is used to detect specific antibody against foot and mouth disease virus (FMDV) Type A Antibody in bovine serum qualitatively, for monitoring antibody after FMDV Type A immune.
This kit use indirect ELISA method, pured FMDV antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is FMD virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-FMD virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.
2. Reagents and contents
Code Item Spec. Code Item Spec.
1 Antigen Coated plates
96 wells 1/2 plate 7 Positive control 0.8/1.6 ml
2 Enzyme Conjugate 6/11 ml 8 Negative control 0.8/1.6 ml
3 10X Washing buffer 50/100 ml 9 Serum dilute plate 1/2 plate
4 Substrate 11/22 ml 10 Adhesive Foil 2/4 pieces
5 Sample diluent 50/100 ml 11 Instruction 1 piece
6 Stop solution 6/11 ml
3. Material required not provided
1) Micropipette: 0.5-10ul, 10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells with 450/630nm wavelenth.
5) Distilled water or deionized water.
6) Microplate Washer
4. Sample preparation
Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis
5.Washing buffer preparation
Return 10X Concentrated washing buffer into room temperature before use, is there is salt crystals, shake to make it dissovled, then dilute it at 10 times with distilled water or deionized water. The diluted washing buffer can store at 4℃ for about 1 week.