Erythromycin ELISA Test Kit

Erythromycin ELISA Test Kit
Catalog No. LSY-10050
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Erythromycin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Erythromycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Erythromycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Erythromycin in it. This value is compared to the standard curve and the Erythromycin concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.2 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Raw milk about 10ppb
Honey (method 1) about 4ppb
Honey (method 2) about 0.2ppb
Note: ppb=ng/mL or ng/g
Cross-reaction rate:
Erythromycin 100%
Recovery rate:
90%±30%

3. Components
1）Micro-well strips: 12 strips with 8 removable wells each
2）Standard solution (1ml/bottle): 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb.
3）Enzyme conjugate (7 mL)
4）Substrate A (7 mL)
5）Substrate B (7 mL)
6）Stop solution (7 mL)
7）20× concentrated washing buffer (30 mL)
8）1× redissolving solution (50 mL)