Doxycycline ELISA test kit

Doxycycline ELISA Test Kit
Catalog No. LSY-10051
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Doxycycline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Doxycycline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Doxycycline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Doxycycline in it. This value is compared to the standard curve and the Doxycycline concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 2 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue about 30ppb
Honey about 50ppb
Milk about 100ppb
Note: ppb=ng/mL or ng/g
Cross-reaction rate:
Doxycycline 100%
Chlortetracycline 400%
Oxytetracyline 200%
Tetracycline 300%
Recovery rate:
Tissue 90±15%

3. Components
1）Micro-well strips: 12 strips with 8 removable wells each
2）10× concentrated standard solution: 0 ppb, 20ppb, 60ppb, 180ppb, 540ppb (0.5ml/bottle)
3）Enzyme conjugate (7 mL)
4）Antibody working solution (7 mL)
5）Substrate A (7 mL)
6）Substrate B (7 mL)
7）Stop solution (7 mL)
8）20× concentrated washing buffer (30 mL)
9）20× concentrated redissolving solution (10 mL)