Diethylstilbestrol(DES) ELISA Test Kit

Diethylstilbestrol ELISA Test Kit
Catalog No. LSY-10022
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Diethylstilbestrol(DES) in the feed, urine, liver, meat, shrimp and fish. The conjugate antigen is pre-coated on the micro-well stripes. The Diethylstilbestrol in the sample competes with the conjugate antigen pre-coated on the micro-well stripes, to interact with the antibodies against Diethylstilbestrol. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the content of Diethylstilbestrol in the sample. This value is compared to the standard curve and the content of the corresponding Diethylstilbestrol is subsequently obtained.

2. Technical specifications
Sensitivity: 0.1 ppb
Detection limit
Shrimp, fish 0.2 ppb
Pork/liver, chicken/liver 2 ppb
Urine 0.6 ppb
Feed 20 ppb
Recovery rate
Urine 70 ±10%
Feed 90 ±10%
Tissue 85 ±10%
Cross-reactions rate
DES 100%
Dienestrol 38.5%
Hexestrol 8.5%
Ethinylestradiol < 0.1%
Estriol < 0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6 × standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb
3） Enzyme conjugate (12 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 5× concentrated redissolving solution (50 mL) transparent cap