Diazepam ELISA Test Kit

Diazepam ELISA Test Kit
Catalog No. LSY-10021
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Diazepam in the feed, urine, liver and meat. The coupling antigen is pre-coated on the micro-well stripes. The Diazepam in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Diazepam antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Diazepam in it. This value is compared to the standard curve and the Diazepam concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.1 ppb
Detection limit
Tissue 1 ppb
Urine 1 ppb
Feed 10 ppb
Recovery rate
Urine 80 ±10%
Feed 75 ±10%
Tissue(meat, liver) 85 ±10%
Cross-reactions rate
Diazepam 100%
Nitrazepam 7.6%
Oxazepam 8.8%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb
3） Enzyme conjugate (12 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 2× concentrated redissolving solution (50 mL) transparent cap