Clenbuterol ELISA Kit

1. Product Description
Clenbuterol belongs to the group of ß-agonists. It has been known that ß-agonists are suitable for use as performance improvers within the field of livestock production; in particular, the meat/fat ratio in fattened animals can be improved or the growth accelerated. However, such compounds have not been authorized as fattening adjuvants up to now.This ELISA KIT is a competitive enzyme immunoassay for the quantitative analysis of clenbuterol in muscle, liver, urine.
2. Test principle
The basis of the test is the antigen-antibody reaction. The microtiter wells are coated with sheep anti- clenbuterol antibodies. Free clenbuterol enzyme conjugate compete for the clenbuterol antibody binding sites. At the same time, the anti- clenbuterol antibodies are also bound by the immobilized capture antibodies. Any unbound enzyme conjugate is then removed in a washing step. Substrate/chromogen is added to the wells and incubated. Bound enzyme conjugate converts the chromogen into a blue product. The addition of the stop solution leads to a color change from blue to yellow. The measurement is made photometrically at 450 nm; the absorption is inversely proportional to the clenbuterol concentration in the sample.