Chromogenic substrate IBX-4041

Name: IBX-4041
BV Blue active ingredient
BVBlue active ingredient
BVBlue substrates
BV Blue substrates
BVBlue¬ substrates

Bacterial Vaginosis Rapid Test Kit core Chromogenic Ingredient IBX-4041;

Chromogenic substrate IBX-4041

chromogenic substrate of sialidase enzyme
Appearance: Light yellow solid powder
Purity: ≥95%
Application: Two-step chromogenic substrates of neuraminidase (sialidase)
Storage: 2-8℃, keep Away from Moisture and light
Packaging details: 1g per bottle or according to customer’s requirement
Several diagnoses exist for bacterial vaginosis, many of which are laborious and require extensive laboratory testing. Predominantly, clinical diagnosis of bacterial vaginosis requires the presence of three out of four criteria elements as described by Amsel: (1) thin, homogeneous, milky vaginal discharge; (2) vaginal-fluid pH greater than 4.5; (3) a positive whiff test (i.e., production of a fishy odor when 10% KOH is added to a slide containing vaginal fluid); and (4) clue cells (greater than 20% of epithelial cells with adherent bacteria) . An alternative diagnostic approach utilizes Gram's staining of vaginal fluid to distinguish normal vaginal flora (i.e. gram-positive rods and lactobacilli) from bacterial vaginosis flora (gram-negative morphotypes). Another alternative approach is based on the detection and measurement of sialidase activity. A commercially available test, BVBlue™, utilizes this approach, wherein a colorimetric substrate changes from yellow to blue upon enzymatic cleavage of ketosidic linkages by sialidases. The BVBlue™ test enables rapid detection of BV in a physician's office, and can thereby provide advantages over the Amsel methods and other methods requiring extensive laboratory testing.

IBX-4041 is the key ingredient of BVBLUE test kit, BVBLUE test is a rapid sialidase test for bacterial vaginosis. BVBLUE test method is currently the only test method approved by the FDA for testing Bacterial Vaginosis(BV), The BV BLUE Test contains a chromogenic substrate of sialidase enzyme, IBX-4041.

BVBLUE Test Kit Component Name
1)BVBLUE Developer Solution: Water and Sodium hydroxide
2) BVBLUE Testing Vessel: Water, Potassium acetate, IBX-4041 (chromogenic substrate compound).

Shenzhen BST Science & Technology Co., Ltd sells and exports chromogenic substrate of sialidase enzyme IBX-4041.
Detection reagent for bacterial vaginosis (sialidase two-step method)

Advantage of BVBLUE Test Kit
compared to other diagnostic test methods for BV such as Amsel criteria, Nugent Gram-stain scoring system, Affirm¬ Microbial Identification Test,
1, BVBLUE Test is currently the only test method approved by the FDA for testing Bacterial Vaginosis(BV).
2, BVBLUE Test just take 10 minutes to 15 minutes to get the result.
3, High purity sialidase substrate with high specificity can reduce the occurrence of false positive.
4. Comparing with the gold standard, the coincidence rate is high, the relative sensitivity is 93%, and the specificity is 95%.
5. Easy to operate, do not need equipment.
6. The specimen does not need to pretreat.

BV Blue test detects sialidase activity, an enzyme produced by BV-associated bacteria such as Gardnerella vaginalis, Bacteroides spp., Prevotella spp., and Mobiluncus spp. In the test procedure, a vaginal fluid sample is placed in the test vessel which contains a chromogenic substrate for sialidase. After incubation, a developer solution is added, and If the sample contained a high level of sialidase, a blue or green color is seen. Samples containing no sialidase, or low levels of this enzyme, will generate a yellow color in the reaction. The BV test has high specificity and sensitivity.