Chlortetracyline ELISA Test Kit

Chlortetracyline ELISA Test Kit
Catalog No. LSY-10049
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chlortetracyline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Chlortetracyline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Chlortetracyline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chlortetracyline in it. This value is compared to the standard curve and the Chlortetracyline concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.2 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue about 3ppb
Cross-reaction rate:
Chlortetracyline 100%
Tetracyclines 80%
Oxytetracyline 40%
Doxycycline 8%
Recovery rate:
Tissue 90±15%

3. Components
1）Micro-well strips: 12 strips with 8 removable wells each
2）10× concentrated standard solution (0.5ml/bottle): 0ppb, 2ppb, 6ppb, 18ppb, 54ppb
3）Enzyme conjugate (6 mL)
4）Antibody working solution (6 mL)
5）Substrate A (6 mL)
6）Substrate B (6 mL)
7）Stop solution (7 mL)
8）20× concentrated washing buffer (30 mL)
9）20× concentrated redissolving solution (10 mL)