Chloramphenicol ELISA test Kit

1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.

2. Technical specifications
Sensitivity: 0.05 ppb
Detection limit:
Intestine 0.1 ppb
Honey 0.1 ppb
Milk 0.05 ppb
Urine, serum 0.1 ppb
Tissue, liver 0.025 ppb
Feed…………………………………………………………………………0.15 ppb
Recovery rate
Tissue, liver 80±10%
Honey, intestine 70±10%
Milk, feed 85±15%
Urine, serum…………………………………………………………………70±10%
Cross-reaction rate:
Chloramphenicol 100%
Thiamphenicol < 0.1%
Florfeniol < 0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb
3） Enzyme conjugate (12 mL) red cap
4） Concentrated antibody working solution (1 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 2× concentrated redissolving solution (50 mL) transparent cap