Canine Heartworm Ag Test

INTENDED USE

Quicking Canine Heartworm Ag Rapid Test is a sandwich lateral flow immunochromatographic assay for the qualitative detection of Dirofilaria immitis (CHW Ag) in dog’s blood, serum or plasma.

Assay Time: 5-10 min
Sample: serum, plasma or whole blood


PRINCIPLE OF THE ASSAY

Quicking Canine Heartworm Ag Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is enough CHW antigen in the sample, a visible T band will appear. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Dirofilaria immitis antigen in the sample.


KIT COMPONENT

10×foil pouches, each containing one cassette, one pipette and a desiccant
10×assay buffer tubes (0.5 mL each)
10×centrifugal tubes
Product Manual


TEST PROCEDURE

Take dog’s blood, serum or plasma as sample.
Take out the cassette from the foil pouch and place it horizontally.
Drip 1 drop of sample and immediately drip 2 drops of assay buffer into the sample hole. NOTE: It is suggested to use serum to increase the detection sensitivity.
Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.


INTERPRETATION OF RESULTS

Positive: The presence of both C band and T band, no matter T band is clear or vague.
Negative: Only clear C band appears.
Invalid: No colored band appears in C zone, no matter whether T band appears.


STORAGE

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.