BlueGene Biotech

Bovine Carnitine Palmitoyltransferase I ELISA kit
Metabolism
E11C0055 Bovine Carnitine Palmitoyltransferase I ELISA kit

Bovine Carnitine Palmitoyltransferase I ELISA kit is suitable for the detection of samples from Bovine species. Carnitine palmitoyltransferase I can also be called arnitine acyltransferase I, CPTI, CAT1, CoA, carnitine acyltransferase i (CCAT), or palmitoyl CoA transferase I, CPT 1, CPT-1.
Specifications of Bovine Carnitine Palmitoyltransferase I ELISA kit
Product Information
Cat. No. E11C0055
Product Name Bovine Carnitine palmitoyltransferase I ELISA kit
Species Bovine
Product Size 48 Tests / 96 Tests
Concentration 2.5-50 ng/mL
Sensitivity 0.1 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 2.5 ng/mL 1 vial
STANDARD C (0.5mL) 5 ng/mL 1 vial
STANDARD D (0.5mL) 10 ng/mL 1 vial
STANDARD E (0.5mL) 25 ng/mL 1 vial
STANDARD F (0.5mL) 50 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay
The CPT 1 ELISA kit uses an anti-CPT 1 antibody and a CPT 1-HRP conjugate to perform a competitive enzyme immunoassay. The CPT 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT 1 concentration since CPT 1 from samples and CPT 1-HRP conjugate compete for the anti-CPT 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT 1 from the sample, fewer sites are left to bind CPT 1-HRP conjugate. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. From this standard curve, the CPT 1 concentration in each sample is extrapolated.

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