BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit

E14C0004 Sheep Cluster of Differentiation 4 ELISA kit

Sheep Cluster of Differentiation 4 ELISA kit is suitable for the detection of samples from sheep species. Cluster of Differentiation 4 can also be called OKT4D, IMD79, CD4 (L3T4), CD4 antigen (p55), CD4 Antigen, CD4 molecule, CD4 Receptor, CD4+ Lymphocyte deficiency, included, CD4mut.
Specifications of Sheep Cluster of Differentiation 4 ELISA kit
Product Information
Cat. No. E14C0004
Product Name Sheep Cluster of Differentiation 4 ELISA kit
Species Sheep
Product Size 48 Tests / 96 Tests
Concentration 25-500 ng/ml
Sensitivity 1.0 ng/mL
Principal Sandwich ELISA
Sample Volume 50 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 10 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 25 ng/mL 1 vial
STANDARD C (0.5mL) 50 ng/mL 1 vial
STANDARD D (0.5mL) 100 ng/mL 1 vial
STANDARD E (0.5mL) 250 ng/mL 1 vial
STANDARD F (0.5mL) 500 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

CD4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CD4. Standards or samples are then added to the microtiter plate wells and CD4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CD4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CD4 are added to each well to "sandwich" the CD4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD4 concentration in each sample is interpolated from this standard curve.


Quality Control On Sheep CD4 ELISA Kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 87-118.37%
Linearity Diluent Ratio Range %
1:2 98-117
1:4 93-106
1:8 91-105
Specificity/Cross-reactivity No significant cross-reactivity or interference between CD4 and analogues was observed.

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