BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit

Specifications of Sheep Anti Interleukin 10 ELISA kit

Product Information
Cat. No. E14A0081
Product Name Sheep Anti Interleukin 10 ELISA kit
Species Sheep
Product Size 48 Tests / 96 Tests
Concentration 25-500 ng/ml
Sensitivity 1.0 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 25 ng/mL 1 vial
STANDARD C (0.5mL) 50 ng/mL 1 vial
STANDARD D (0.5mL) 100 ng/mL 1 vial
STANDARD E (0.5mL) 250 ng/mL 1 vial
STANDARD F (0.5mL) 500 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay
Anti-il 10 elisa applies the competitive enzyme immunoassay technique utilizing IL 10 antigen and an Anti-IL 10-HRP conjugate. The assay sample and buffer are incubated together with Anti-IL 10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-IL 10 concentration since Anti-IL 10 from samples and Anti-IL 10-HRP conjugate compete for the IL 10 antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-IL 10 from the sample, fewer sites are left to bind Anti-IL 10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-IL 10 concentration in each sample is interpolated from this standard curve.

Quality Control on Sheep Anti Interleukin 10 ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 87-118.37%
Linearity Diluent Ratio Range %
1:2 98-117
1:4 93-106
1:8 91-105
Specificity/Cross-reactivity No significant cross-reactivity or interference between Anti IL 10 and analogues was observed.

Bluegene Biotech has been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the advantages of high specificity, high sensitivity, and high stability.

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