BlueGene Biotech Canine Ferritin ELISA kit

E08F0010 Canine Ferritin ELISA kit

Canine Ferritin ELISA kit is suitable for the detection of samples from Canine species. Ferritin can also be called FE.

Specifications of Canine Ferritin ELISA Test kit
Product Information
Cat. No. E08F0010
Product Name Canine Ferritin ELISA kit
Species Canine
Product Size 48 Tests / 96 Tests
Concentration 25-500 ng/mL
Sensitivity 1.0 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 25 ng/mL 1 vial
STANDARD C (0.5mL) 50 ng/mL 1 vial
STANDARD D (0.5mL) 100 ng/mL 1 vial
STANDARD E (0.5mL) 250 ng/mL 1 vial
STANDARD F (0.5mL) 500 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

FE ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FE antibody and an FE-HRP conjugate. The assay sample and buffer are incubated together with FE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FE concentration since FE from samples and FE-HRP conjugate compete for the anti-FE antibody binding site. Since the number of sites is limited, as more sites are occupied by FE from the sample, fewer sites are left to bind FE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FE concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Ferritin ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 92-108%
Linearity Diluent Ratio Range %
1:02 87-113
1:04 82-116
1:08 84-115
Specificity/Cross-reactivity No significant cross-reactivity or interference between FE and analogues was observed.

Now the ferritin elisa kit price is reasonable, if you have needs, please contact us.

Bluegene Biotech has been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the advantages of high specificity, high sensitivity, and high stability.

If you want to know more kinds of host cell protein elisa kits, please visit our website.