BlueGene Biotech Canine Endothelin 1 ELISA kit

E08E0040 Canine Endothelin 1 ELISA kit

Canine Endothelin 1 ELISA kit is suitable for the detection of samples from Canine species. Endothelin 1 can also be called Endothelin-1, Big endothelin 1, EDN1 protein, Endothelin1, ET1, PPET1, Preproendothelin 1.

Specifications of Canine Endothelin ELISA kit
Product Information
Cat. No. E08E0040
Product Name Canine Endothelin 1 ELISA kit
Species Canine
Product Size 48 Tests / 96 Tests
Concentration 0.5-10 ng/mL
Sensitivity 0.1 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 0.5 ng/mL 1 vial
STANDARD C (0.5mL) 1 ng/mL 1 vial
STANDARD D (0.5mL) 2.5 ng/mL 1 vial
STANDARD E (0.5mL) 5 ng/mL 1 vial
STANDARD F (0.5mL) 10 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

Big Endothelin 1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EDN1 antibody and an EDN1-HRP conjugate. The assay sample and buffer are incubated together with EDN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EDN1 concentration since EDN1 from samples and EDN1-HRP conjugate compete for the anti-EDN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by EDN1 from the sample, fewer sites are left to bind EDN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EDN1 concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Endothelin 1 ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 89-108%
Linearity Diluent Ratio Range %
1:02 86-105
1:04 85-110
1:08 82-112
Specificity/Cross-reactivity No significant cross-reactivity or interference between EDN1 and analogues was observed.

Bluegene Biotech has been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the advantages of high specificity, high sensitivity, and high stability.

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