BlueGene Biotech Canine Collagen Type II Alpha 1 ELISA kit

E08C0323 Canine Collagen Type II Alpha 1 ELISA kit

Canine Collagen Type II Alpha 1 ELISA kit is suitable for the detection of samples from Canine species. Collagen Type II Alpha 1 can also be called COL2-A1, COL2A1, COL2 A1, SEDC, Chondrocalcin, COL2α1, COL2-α1, COL2 α1.

Specifications of Canine Collagen Type II Alpha 1 ELISA kit
Product Information
Cat. No. E08C0323
Product Name Canine Collagen Type II Alpha 1 ELISA kit
Species Canine
Product Size 48 Tests / 96 Tests
Concentration 10-250 ng/mL
Sensitivity 1.0 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 10 ng/mL 1 vial
STANDARD C (0.5mL) 25 ng/mL 1 vial
STANDARD D (0.5mL) 50 ng/mL 1 vial
STANDARD E (0.5mL) 100 ng/mL 1 vial
STANDARD F (0.5mL) 250 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

COL2a1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-COL2a1 antibody and an COL2a1-HRP conjugate. The assay sample and buffer are incubated together with COL2a1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL2a1 concentration since COL2a1 from samples and COL2a1-HRP conjugate compete for the anti-COL2a1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL2a1 from the sample, fewer sites are left to bind COL2a1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL2a1 concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Collagen Type II Alpha 1 ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 87-104%
Linearity Diluent Ratio Range %
1:02 87-105
1:04 84-109
1:08 81-108
Specificity/Cross-reactivity No significant cross-reactivity or interference between COL2a1 and analogues was observed.

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