BlueGene Biotech Canine β Cell Leukemia/Lymphoma 2 ELISA kit

E08B0033 Canine β Cell Leukemia/Lymphoma 2 ELISA kit

Canine β Cell Leukemia/Lymphoma 2 ELISA kit is suitable for the detection of samples from Canine species. β Cell Leukemia/Lymphoma 2 can also be called BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4, BCL 2, B-cell leukemia/lymphoma 2, Bcl-2 alpha, Apoptosis regulator Bcl-2, Bcl-2-like protein 1, Bcl-2-associated X protein, Bcl-2 homologous antagonist/killer, Bcl-2-related ovarian killer protein, Bcl-w, Bcl-xL, Bcl-xS, Protein phosphatase 1 regulatory subunit 52, PPP1R5.

Specifications of Canine β Cell Leukemia/Lymphoma 2 ELISA kit
Product Information
Cat. No. E08B0033
Product Name Canine β Cell Leukemia/Lymphoma 2 ELISA kit
Species Canine
Product Size 48 Tests / 96 Tests
Concentration 10-25 ng/mL
Sensitivity 0.1 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 0.5 ng/mL 1 vial
STANDARD C (0.5mL) 1 ng/mL 1 vial
STANDARD D (0.5mL) 2.5 ng/mL 1 vial
STANDARD E (0.5mL) 5 ng/mL 1 vial
STANDARD F (0.5mL) 10 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

BCL2 ELISA applies the competitive enzyme immunoassay technique utilizing an anti-BCL 2 antibody and an BCL 2-HRP conjugate. The assay sample and buffer are incubated together with BCL 2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL 2 concentration since BCL 2 from samples and BCL 2-HRP conjugate compete for the anti-BCL 2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL 2 from the sample, fewer sites are left to bind BCL 2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL 2 concentration in each sample is interpolated from this standard curve.

Quality Control on Canine β Cell Leukemia/Lymphoma 2 ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 86-107%
Linearity Diluent Ratio Range %
1:02 86-109
1:04 83-111
1:08 83-115
Specificity/ Cross-reactivity No significant cross-reactivity or interference between BCL 2 and analogues was observed.

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