BlueGene Biotech Canine Caspase 3 ELISA kit

E08C0549 Canine Caspase 3 ELISA kit

Canine Caspase 3 ELISA kit is suitable for the detection of samples from Canine species. Caspase 3 can also be called CPP32,CASP3B,SCA1,Apoptain, CASP3.

Specifications of Canine Caspase 3 ELISA kit
Product Information
Cat. No. E08C0549
Product Name Canine Caspase 3 ELISA kit
Species Canine
Product Size 48 Tests / 96 Tests
Concentration 0.5-10 ng/mL
Sensitivity 0.1 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 0.5 ng/mL 1 vial
STANDARD C (0.5mL) 1 ng/mL 1 vial
STANDARD D (0.5mL) 2.5 ng/mL 1 vial
STANDARD E (0.5mL) 5 ng/mL 1 vial
STANDARD F (0.5mL) 10 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

CASP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CASP3 antibody and an CASP3-HRP conjugate. The assay sample and buffer are incubated together with CASP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASP3 concentration since CASP3 from samples and CASP3-HRP conjugate compete for the anti-CASP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CASP3 from the sample, fewer sites are left to bind CASP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP3 concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Caspase 3 Insulin ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 88-102%
Linearity Diluent Ratio Range %
1:02 86-103
1:04 83-108
1:08 81-107
Specificity/Cross-reactivity No significant cross-reactivity or interference between CASP3 and analogues was observed.

Shanghai Bluegene Biotech has been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the advantages of high specificity, high sensitivity, and high stability.

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