BlueGene Biotech Canine BCL2 Associated X Protein ELISA kit

Shanghai BlueGene Biotech
Canine BCL2 Associated X Protein ELISA kit
E08B0032 Canine BCL2 Associated X Protein ELISA kit

The Canine BCL2 Associated X Protein ELISA kit is suitable for the detection of samples from Canine species. BCL2 Associated X Protein can also be called BAX, BCL2L4, Bax Zeta, Apoptosis regulator.

Specifications of Canine BCL2 Associated X Protein ELISA kit
Product Information
Cat. No. E08B0032
Product Name Canine BCL2 Associated X Protein ELISA kit
Species Canine
Product Size 48 Tests / 96 Tests
Concentration 5-100 ng/mL
Sensitivity 1.0 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 5 ng/mL 1 vial
STANDARD C (0.5mL) 10 ng/mL 1 vial
STANDARD D (0.5mL) 25 ng/mL 1 vial
STANDARD E (0.5mL) 50 ng/mL 1 vial
STANDARD F (0.5mL) 100 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

BAX ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BAX antibody and an BAX-HRP conjugate. The assay sample and buffer are incubated together with BAX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAX concentration since BAX from samples and BAX-HRP conjugate compete for the anti-BAX antibody binding site. Since the number of sites is limited, as more sites are occupied by BAX from the sample, fewer sites are left to bind BAX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAX concentration in each sample is interpolated from this standard curve.

Quality Control on Canine BCL2 Associated X Protein ELISA BAX kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 90-104%
Linearity Diluent Ratio Range %
1:02 89-105
1:04 87-106
1:08 84-109
Specificity/Cross-reactivity No significant cross-reactivity or interference between BAX and analogues was observed.

Citations of Canine BCL2 Associated X Protein ELISA kit
E08B0032 has been referenced in the below publications:

Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog.

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