BlueGene Biotech Bovine Catalase ELISA kit

E11C0086 Bovine Catalase ELISA kit

Bovine Catalase ELISA kit is suitable for the detection of samples from Bovine species. Catalase can also be called Cas1, CAT, Cs1.

Specifications of Bovine Catalase ELISA kit
Product Information
Cat. No. E11C0086
Product Name Bovine Catalase ELISA kit
Species Bovine
Product Size 48 Tests / 96 Tests
Concentration 10-250 ng/mL
Sensitivity 1.0 ng/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8®C

Kit Components
MATERIALS SPECIFICATION QUANTITY
MICROTITER PLATE 96 wells stripwell
ENZYME CONJUGATE 6.0 mL 1 vial
STANDARD A (0.5mL) 0 ng/mL 1 vial
STANDARD B (0.5mL) 10 ng/mL 1 vial
STANDARD C (0.5mL) 25 ng/mL 1 vial
STANDARD D (0.5mL) 50 ng/mL 1 vial
STANDARD E (0.5mL) 100 ng/mL 1 vial
STANDARD F (0.5mL) 250 ng/mL 1 vial
SUBSTRATE A 6 mL 1 vial
SUBSTRATE B 6 mL 1 vial
STOP SOLUTION 6 mL 1 vial
WASH SOLUTION (100 x) 10 mL 1 vial
BALANCE SOLUTION 3 mL 1 vial

Principle of the Assay

CAT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CAT antibody and an CAT-HRP conjugate. The assay sample and buffer are incubated together with CAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAT concentration since CAT from samples and CAT-HRP conjugate compete for the anti-CAT antibody binding site. Since the number of sites is limited, as more sites are occupied by CAT from the sample, fewer sites are left to bind CAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAT concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine Catalase ELISA kit


Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 91-105%
Linearity Diluent Ratio Range %
1:2 87-108
1:4 89-109
1:8 85-111
Specificity/Cross-reactivity No significant cross-reactivity or interference between CAT and analogues was observed.

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