Benzyl penicillin ELISA test kit

Benzyl penicillin ELISA Test Kit
Catalog No. LSY-10019
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Benzyl penicillin in samples. The antigens conjugated Benzyl penicillin is pre-coated on the micro-well stripes, Benzyl penicillin in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti-Benzyl penicillin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Benzyl penicillin concentration in the sample. This value is compared to the standard curve and concentration of Benzyl penicillin in the sample is subsequently obtained.

2. Technical specifications
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 30min~30min~15min
Detection limit:
Tissue, honey, milk 2 ppb
Because of some interference, quantification limit of honey, milk sample is 4 ppb
Recovery rate:
Tissue 75±19%
Honey, milk 70±17%
Cross-reaction rate:
Benzyl penicillin 100%
Ampicillin 0.8%
Cloxacillin 0.2%
Dicloxacillin 0.1%
Amoxicillin 0.1%
Ceftiofu 0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb
3） Enzyme conjugate (12 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 5× concentrated redissolving solution (50 mL) transparent cap