Anti-pseudorabies virus ge protein ELISA kit

Anti-pseudorabies virus antibody ELISA kit
Porcine Pseudorabies virus antibody Rapid Assay
Catalog No. PRV041117A
Summary
Porcine Pseudorabies, is a contagion caused by Porcine Pseudorabies virus (PRV), which lead to misbirth, stillbirth and mummy fetus in gestation of sow, high mortality of baby pig and unwonted respiration. Detection of specific antibodies against PRV can be indicators of vaccination status and assistant diadynamic criteria.
This kit use PRV solid phase antigen to detect specific antibody against PRRS antigen, have high sensitivity, specificity, reproducibility, and is easy to operate quickly and can be used in multiple times.
Reagents and contents
1 8ÿ?Coated microtiter strips: ready to use
2 1ÿ?Conjugate solution: ready to use
3 1ÿ?Washing solution: ready to use
4 1ÿ?Substrate A solution: ready to use
5 1ÿ?Substrate B solution: ready to use
6 1ÿ?Sample dilution: ready to use
7 1ÿ?Stopping solution: ready to use
Warning: Stopping solution irritates eyes and skin. Keep out of the reach of children. Upon contact with the eyes, rinse thoroughly with water and consult a doctor.
8 1ÿ?Positive control: ready to use
9 1ÿ?Negative control: ready to use
10 1ÿ?Instruction sheet
Test procedure
1 Sample preparation:
Dilutes the patientÿ®s serum for the test with sample dilution at 1:100, e.g. 5 ¥Ìl serum + 495¥Ìl sample dilution, and mix thoroughly.
2 Adding samples and controls
Leave well A1 for reagent blank. Pipette controls and samples as follows:
100¥Ìl of negative control and positive control respectively, and 100¥Ìl diluted samples each into remaining wells.
Incubation at 37ÿæfor 30minutes. Discard off contents of the wells and fill all wells with washing solution completely, incubate for 1 min and discard off. Perform another 2 washing cycles as above. At the end of the washing step carefully remove remaining fluid by tapping the strips on the tissue paper prior to the next step.
3 Adding conjugate solution
Add 1 drop into all wells and incubate at 37ÿ?for 30 minutes. Discard the contents of the wells and wash 3 times as described in step 2.
4 Adding substrates
Add one drop of substrate A solution and B solution respectively, incubate at 37ÿ?for 10 minutes.
Add one drop of stopping solution into all wells. Zero the ELISA microtiter plate reader using the reagent blank well A1. Measure the absorbance at 450 nm.
Results
Judgment by microtiter plate reader
Cut-off value = 2.1ÿÁA- (absorbance of negative control)
Interpretation of results
A450 (patient) ÿ?cut-off: positive
A450 (patient) ¡¼ cut-off: negative
Notes
1 store at 2-8, make the temperature of the kit return to room temperature before use. screw down the cap after use, don't mix caps between diffirent bottles and don't mix components between diffirent kits
2 recommand to judge results with instruments to improve comparability of testing.