Ampicillin ELISA Test Kit

Ampicillin ELISA Test Kit
Catalog No. LSY-10018
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Ampicillin in samples. The antigens conjugated Ampicillin is pre-coated on the micro-well stripes, Ampicillin in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti-Ampicillin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Ampicillin concentration in the sample. This value is compared to the standard curve and concentration of Ampicillin in the sample is subsequently obtained.

2. Technical specifications
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 30min~30min~15min
Detection limit:
Tissue, honey, milk 2 ppb
Because of some interference, quantification limit of honey, milk sample is 4 ppb
Cross-reaction rate:
Ampicillin 100%
Benzyl penicillin 0.8%
Cloxacillin 0.2%
Dicloxacillin 0.1%
Amoxicillin 0.1%
Ceftiofu 0.1%
Recovery rate:
Tissue 75±19%
Honey, milk 70±17%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb
3） Enzyme conjugate (12 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 5× concentrated redissolving solution (50 mL) transparent cap