Amantadine ELISA test kit

Amantadine ELISA Test Kit
Catalog No. LSY-10052
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Amantadine. The coupling antigen is pre-coated on the micro-well stripes. The Amantadine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Amantadine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Amantadine in the sample. This value is compared to the standard curve and the Amantadine residues is subsequently obtained.

2. Technical specifications
Sensitivity: 0.2 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit:
Chicken, duck……………………………………….............…………….0.2ppb
Cross-reaction rate:
Amantadine 100%
Recovery rate:
Chicken, duck……………………………………….......................……90±25%

3.Components
1 Micro-well strips 12 strips with 8 removable wells each
2 6× standard solution (1 mL each) 0ppb 0.2ppb
0.8ppb 3.2ppb
12.8ppb 51.2ppb
3 Enzyme conjugate 7ml red cap
4 Antibody working solution 7ml blue cap
5 Substrate A 7ml white cap
6 Substrate B 7ml black cap
7 Stop solution 7ml yellow cap
8 Extractant 50ml*2 transparent cap
9 20× concentrated washing buffer 15ml white cap
10 5× concentrated redissolving solution 10ml white cap